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| Content Provider | PubMed Central |
|---|---|
| Author | Zhang, Hao Xu, Zimu Shen, Jie Li, Xu Ding, Lili Ma, Jie Lan, Yan Xia, Weidong Cheng, Cheng Sun, Qiang Zhang, Zelong Chu, Paul K. |
| Copyright Year | 2015 |
| Abstract | Proteins are carriers of biological functions and the effects of atmospheric-pressurenon-thermal plasmas on proteins are important to applications such as sterilizationand plasma-induced apoptosis of cancer cells. Herein, we report our detailedinvestigation of the effects of helium-oxygen non-thermal dielectric barrierdischarge (DBD) plasmas on the inactivation of lactate dehydrogenase (LDH) enzymesolutions. Circular dichroism (CD) and dynamic light scattering (DLS) indicate thatthe loss of activity stems from plasma-induced modification of the secondarymolecular structure as well as polymerization of the peptide chains. Raising thetreatment intensity leads to a reduced alpha-helix content, increase in thepercentage of the beta-sheet regions and random sequence, as well as graduallydecreasing LDH activity. However, the structure of the LDH plasma-treated for 300seconds exhibits a recovery trend after storage for 24 h and itsactivity also increases slightly. By comparing direct and indirect plasmatreatments, plasma-induced LDH inactivation can be attributed to reactive species(RS) in the plasma, especially ones with a long lifetime including hydrogenperoxide, ozone, and nitrate ion which play the major role in the alteration of themacromolecular structure and molecular diameter in lieu of heat, UV radiation, andcharged particles. |
| Related Links | http://dx.doi.org/10.1038/srep10031 |
| Starting Page | 10031 |
| File Format | |
| ISSN | 20452322 |
| e-ISSN | 20452322 |
| Journal | Scientific Reports |
| Volume Number | 5 |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2015-05-01 |
| Access Restriction | Open |
| Rights Holder | Nature Publishing Group |
| Subject Keyword | Science and technology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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