NDLI: Compaction of rolling circle amplification products increases signal integrity and signal-to-noise ratio

Content Provider	PubMed Central
Author	Clausson, Carl-magnus Linda, Arngården Ishaq, Omer Axel, Klaesson Kühnemund, Malte Karin, Grannas Koos, Björn Qian, Xiaoyan Petter, Ranefall Tomasz, Krzywkowski Brismar, Hjalmar Nilsson, Mats Carolina, Wählby Söderberg, Ola
Copyright Year	2015
Abstract	Rolling circle amplification (RCA) for generation of distinct fluorescent signals in situ relies upon the self-collapsing properties of single-stranded DNA in commonly used RCA-based methods. By introducing a cross-hybridizing DNA oligonucleotide during rolling circle amplification, we demonstrate that the fluorophore-labeled RCA products (RCPs) become smaller. The reduced size of RCPs increases the local concentration of fluorophores and as a result, the signal intensity increases together with the signal-to-noise ratio. Furthermore, we have found that RCPs sometimes tend to disintegrate and may be recorded as several RCPs, a trait that is prevented with our cross-hybridizing DNA oligonucleotide. These effects generated by compaction of RCPs improve accuracy of visual as well as automated in situ analysis for RCA based methods, such as proximity ligation assays (PLA) and padlock probes.
Related Links	http://dx.doi.org/10.1038/srep12317
Starting Page	12317
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	5
Language	English
Publisher	Nature Publishing Group
Publisher Date	2015-07-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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