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Content Provider | PubMed Central |
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Author | Taeger, Christian D. Friedrich, Oliver Dragu, Adrian Weigand, Annika Hobe, Frieder Drechsler, Caroline Geppert, Carol I. Andreas, Arkudas Münch, Frank Buchholz, Rainer Pollmann, Charlotte Schramm, Axel Birkholz, Torsten Horch, Raymund E. Konstantin, Präbst |
Copyright Year | 2015 |
Abstract | Preventing ischemia-related cell damage is a priority when preserving tissue for transplantation. Perfusion protocols have been established for a variety of applications and proven to be superior to procedures used in clinical routine. Extracorporeal perfusion of muscle tissue though cumbersome is highly desirable since it is highly susceptible to ischemia-related damage. To show the efficacy of different perfusion protocols external field stimulation can be used to immediately visualize improvement or deterioration of the tissue during active and running perfusion protocols. This method has been used to show the superiority of extracorporeal perfusion using porcine rectus abdominis muscles perfused with heparinized saline solution. Perfused muscles showed statistically significant higher ability to exert force compared to nonperfused ones. These findings can be confirmed using Annexin V as marker for cell damage, perfusion of muscle tissue limits damage significantly compared to nonperfused tissue. The combination of extracorporeal perfusion and external field stimulation may improve organ conservation research. |
Related Links | http://dx.doi.org/10.1038/srep11956 |
Starting Page | 11956 |
File Format | |
ISSN | 20452322 |
e-ISSN | 20452322 |
Journal | Scientific Reports |
Volume Number | 5 |
Language | English |
Publisher | Nature Publishing Group |
Publisher Date | 2015-07-01 |
Access Restriction | Open |
Rights Holder | Nature Publishing Group |
Subject Keyword | Science and technology Research in Higher Education |
Content Type | Text |
Resource Type | Article |
Subject | Multidisciplinary |
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