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Content Provider | PubMed Central |
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Author | Ahmed, Usman Anwar, Attia Savage, Richard S. Costa, Matthew L. Mackay, Nicola Filer, Andrew Raza, Karim Watts, Richard A. Winyard, Paul G. Tarr, Joanna Haigh, Richard C. Thornalley, Paul J. Rabbani, Naila |
Copyright Year | 2015 |
Abstract | There is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests for early-stage rheumatoid arthritis (eRA) such as rheumatoid factor (RF) and anti–cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass spectrometric methods to quantify citrullinated protein (CP) and free hydroxyproline in body fluids. We detected CP in the plasma of healthy subjects and surprisingly found that CP was increased in both patients with eOA and eRA whereas anti–CCP antibodies were predominantly present in eRA. A 4-class diagnostic algorithm combining plasma/serum CP, anti-CCP antibody and hydroxyproline applied to a cohort gave specific and sensitive detection and discrimination of eOA, eRA, other non-RA inflammatory joint diseases and good skeletal health. This provides a first-in-class plasma/serum-based biochemical assay for diagnosis and type discrimination of early-stage arthritis to facilitate improved treatment and patient outcomes, exploiting citrullinated protein and related differential autoimmunity. |
Related Links | http://dx.doi.org/10.1038/srep09259 |
Starting Page | 9259 |
File Format | |
ISSN | 20452322 |
e-ISSN | 20452322 |
Journal | Scientific Reports |
Volume Number | 5 |
Language | English |
Publisher | Nature Publishing Group |
Publisher Date | 2015-03-01 |
Access Restriction | Open |
Rights Holder | Nature Publishing Group |
Subject Keyword | Science and technology Research in Higher Education |
Content Type | Text |
Resource Type | Article |
Subject | Multidisciplinary |
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