NDLI: High resolution μSPECT for brain activation analysis in small animals

Content Provider	IEEE Xplore Digital Library
Author	Staelens, S. Wyckhuys, T. Deleye, S. Hallez, H. Vandenberghe, S. Van Nieuwenhuyse, B. Vonck, K.
Copyright Year	2009
Description	Author affiliation: Medical Signal and Image Processing group, Ghent University - IBBT, Belgium (Staelens, S.; Wyckhuys, T.; Deleye, S.; Hallez, H.; Vandenberghe, S.) \|\| Laboratory for Clinical and Experimental Neurophysiology, Department of Neurology, Ghent University Hospital, Belgium (Van Nieuwenhuyse, B.; Vonck, K.)
Abstract	A stimulus on/off imaging study is often used to evaluate the brain's response to a presented visual, electrical or chemical trigger. Clinical software and human templates already exist but given the recent advent of ultrahigh resolution μSPECT and μPET, a larger need rises for a post processing platform to perform these subtraction molecular imaging studies also in small animals. We have designed such a multimodal framework to perform μSPECT activation studies in rats thereby making use of μCT and MRI for anatomical land marking. Our software solution is a combination of Amide, MRIcroN and a custom made Matlab implementation. We have studied the performance of different deep brain stimulations for which 6 rats were implanted with a multi-polar stimulation electrode in the right hippocampus. Each animal underwent a 99mTc-HMPAO μSPECT with the Milabs U-SPECT-II and a μCT scan with the GMI X-O CT before and after stimulation. Two line markers in oblique positions filled with low activity of 125I are used to register the μSPECT and μCT images. Afterwards the animals were sacrificed, their electrode was removed and a MRI scan was performed using the wrist coil of a Siemens Trio 3T. A semi-automated five step procedure delivers the activation map: (i) first the stimulus-on μCT and the MRI are registered to the stimulus-off μCT followed by (ii) the fusion of the off/on μSPECT scans with their off/on μCT counterparts. From the MRI, (iii) the rat brain is extracted, which is used as a mask for the calculation. Afterwards, (iv) both off/on μSPECT scans are normalized and subtracted within this MRI brain mask. Finally, (v) the Z-score, representing the activation map, is achieved by dividing the result with the standard deviation of the masked stimulus-off μSPECT. This semi-automated approach allows the experimental neu-roscientist to draw conclusions on the location, spatial extent and intensity of the small animal brains response to the stimulus.
Starting Page	2702
Ending Page	2705
File Size	876895
Page Count	4
File Format	PDF
ISBN	9781424439614
ISSN	10957863
DOI	10.1109/NSSMIC.2009.5401980
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-10-24
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Activation analysis Animals Computed tomography Magnetic resonance imaging Rats Electrodes High-resolution imaging Chemicals Software performance Humans voxel based μ SPECT activation study multimodal
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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