NDLI: Electro-Disruption of Escherichia coli Bacterial Cells on a Microfabricated Chip

Content Provider	IEEE Xplore Digital Library
Author	de la Rosa, C. Kaler, K.V.I.S.
Copyright Year	2006
Description	Author affiliation: Dept. of Electr. & Comput; Eng., Calgary Univ., Alta. (de la Rosa, C.; Kaler, K.V.I.S.)
Abstract	A miniaturized system for sample preparation of relevant bacterial pathogens has been developed using a variety of microfabrication techniques. The system manipulates and disrupts Eschericha coli bacterial cells using dielectrophoresis, electroporation and enzymes. The microchip consisted of circular gold electrodes patterned on glass using standard photolithography housed in a PDMS chamber. The bacterial lysis efficiency by electroporation and enzymatic degradation was evaluated on the microchip. The miniaturized system was capable of concentrating and aligning bacterial cells in regions of high electric field by dielectrophoresis. The miniaturized sample preparation system had a lysis efficiency of 17% when the bacterial cells were suspended in a 0.25 M sucrose solution and increased to 80% when the bacterial cells were suspended in a solution containing 0.25 M sucrose and 10 KU/ml lysozyme. Sample preparation is a limiting factor for the successful application of molecular pathogen detection methods. Therefore, the development of miniaturized systems useful for sample preparation will improve molecular detection methods of bacterial pathogens
Sponsorship	IEEE EMB
Starting Page	4096
Ending Page	4099
File Size	280486
Page Count	4
File Format	PDF
ISBN	1424400325
ISSN	1557170X
DOI	10.1109/IEMBS.2006.259517
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-08-30
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microorganisms Pathogens Dielectrophoresis Sugar Biochemistry Gold Electrodes Glass Lithography Degradation
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effect of self-assembled monolayers (SAMs) in binding glucose oxidase for electro-enzymatic glucose sensor with gold electrodes

Electrode robustness in artificial cerebrospinal fluid for dielectrophoresis-based LoC

Isolation of rare cancer cells from blood cells using dielectrophoresis

CMOS/microfluidic Lab-on-chip for cells-based diagnostic tools

On-chip electroporation: Characterization, modeling and experimental results

Separatopm method of blood constituents using dielectrophoresis and flow-induced shear force

A low cost solution for the fabrication of dielectrophoretic microfluidic devices and embedded electrodes

A carbon nanotube gas sensor fabricated by dielectrophoresis and its application for NH3 detection

Experimenting with microbial fuel cells for powering implanted biomedical devices

Electro-Disruption of Escherichia coli Bacterial Cells on a Microfabricated Chip

Similar Documents

Effect of self-assembled monolayers (SAMs) in binding glucose oxidase for electro-enzymatic glucose sensor with gold electrodes

Electrode robustness in artificial cerebrospinal fluid for dielectrophoresis-based LoC

Isolation of rare cancer cells from blood cells using dielectrophoresis

CMOS/microfluidic Lab-on-chip for cells-based diagnostic tools

On-chip electroporation: Characterization, modeling and experimental results

Separatopm method of blood constituents using dielectrophoresis and flow-induced shear force

A low cost solution for the fabrication of dielectrophoretic microfluidic devices and embedded electrodes

A carbon nanotube gas sensor fabricated by dielectrophoresis and its application for NH3 detection

Experimenting with microbial fuel cells for powering implanted biomedical devices

Electro-Disruption of Escherichia coli Bacterial Cells on a Microfabricated Chip