NDLI: Towards tissue engineering of meniscus substitutes: selection of cell source and culture environment

Content Provider	IEEE Xplore Digital Library
Author	Marsano, A. Vunjak-Novakovic, G. Martin, I.
Copyright Year	2006
Description	Author affiliation: Dept. of Surg., Basel Univ. Hosp. (Marsano, A.)
Abstract	With the ultimate goal to engineer a meniscus substitute based on autologous cells, we aimed this work at identifying (i) a human cell source capable of generating fibrocartilaginous tissues and (ii) a culture environment promoting the development of bi-zonal constructs, resembling the complex structure and function of a meniscus. The post-expansion differentiation capacity of different chondrogenic cells readily available by knee arthroscopy, namely inner meniscus, fat pad, synovial membrane cells and articular chondrocytes (AC), was assessed within hyaluronan based non-woven meshes. Under our experimental conditions, only expanded AC generated tissues containing relevant amounts of glycosaminoglycans (GAG) and with cell phenotypes compatible with those of the inner and outer meniscus regions. Physical conditioning of constructs generated by expanded AC was applied using mixed flasks. The hydrodynamic environment of mixed flasks was instrumental to promote the formation of bi-zonal tissues, with an inner region rich in GAG and stiffer in compression and an outer rim rich in collagen and stiffer in tension. Therefore, the use of AC cultured within porous scaffolds in mixed flasks allowed engineering of constructs resembling some aspects of the phenotype and function of meniscus tissue
Sponsorship	IEEE EMB
Starting Page	3656
Ending Page	3658
File Size	402981
Page Count	3
File Format	PDF
ISBN	1424400325
ISSN	1557170X
DOI	10.1109/IEMBS.2006.259748
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-08-30
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Tissue engineering Surgery Knee Humans Hydrodynamics Biomembranes AC generators Hospitals Biomedical engineering Flexible printed circuits
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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