NDLI: A 3-D Microelectrodes Array Microchip Applied to Cell Fusion

Content Provider	IEEE Xplore Digital Library
Author	Zhao Zhiqiang Zheng XiaoLin Xiong Jiangong Cui Jianguo Wen Jing Li Zhiguo Wang Xing
Copyright Year	2005
Description	Author affiliation: Coll. of Bioeng., Chongqing Univ. (Zhao Zhiqiang; Zheng XiaoLin; Xiong Jiangong; Cui Jianguo; Wen Jing; Li Zhiguo; Wang Xing)
Abstract	A cell-to-cell fusion technique using 3-D microelectrodes array microchip has been developed. It permits cell-to-cell fusion operation in large amount simultaneously. And it needs much lower voltage (alternating voltage and pulse voltage) than the conventional cell fusion techniques. An array of 16 (4times4) microelectrodes can be fabricated on a 1times1cm silicon wafer. And an experimental system, including circuit control unit, temperature control unit, etc., has been developed to fuse cells. The alternating voltage is applied to the electrodes makes cells in alignment and in pairs firstly. Dielectrophoresis (DEP) force is used to form cell pairs in the microchip and makes cell pairs contact each other tightly. And then fusion of the cell pairs is induced by electric pulse is applied between electrodes. Microelectrodes of 8mum carbon-fiber are used to test the feasibility of the proposal experimental system. Human hepatocarcinoma SMMC-7721 cells are used as specimens. It's observed that SMMC-7721 cells formed cell pairs and fused successfully
Starting Page	1282
Ending Page	1285
File Size	250520
Page Count	4
File Format	PDF
ISBN	0780387414
DOI	10.1109/IEMBS.2005.1616660
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-01-17
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microelectrodes Voltage Temperature control Electrodes Silicon Circuits Control systems Fuses Dielectrophoresis System testing array cell fusion microchip
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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