NDLI: Neurobiochemistry and neuroelectrophysiology of neuron-like cells differentiated from neural BMSCs-D-NSCs

Content Provider	IEEE Xplore Digital Library
Author	Xiaodan Jiang Ruxiang Xu Zaiyu Guo Jianrong Chen Zhanhui Li Yuxi Zou Yingqian Cai Mouxuan Du Zhen Deng Bingxiang Xiao
Copyright Year	2005
Description	Author affiliation: Neuromed. Inst., Southern Medical Univ. (Xiaodan Jiang; Ruxiang Xu; Zaiyu Guo; Jianrong Chen; Zhanhui Li; Yuxi Zou; Yingqian Cai; Mouxuan Du; Zhen Deng; Bingxiang Xiao)
Abstract	In this experiment, the bone marrow stroma cells (BMSCs) were harvested and then cultured in "neural stem cells (NSCs) medium" which was modulated by our lab with R R. of China patent number as ZL 02134314.4 in vitro. The proliferation of NSCs was confirmed by formation of cell's clones and scan electron microscopy test. The identifications of NSCs, neurons-like cells and glial-like cells were immunocytochemically performed by detecting Nestin, neuron-specific enolase (NSE), and glial fibrillary acidic protein (GFAP). Neurons-like cells were further identified by detecting excitability amino acid, inhibited amino acid, or monoamine biological activity materials with high pressure liquid chromatograph (HPLC), and also by examining the electrophysiological properties with patch clamp, in order to verify their neuron-like functions. It was implied that the differentiated BMSCs-NSCs-derived neuron-like cells were characterized by both the neuron-like bio-chemical function and some corresponding electrophysiological properties
Starting Page	5215
Ending Page	5218
File Size	217591
Page Count	4
File Format	PDF
ISBN	0780387414
DOI	10.1109/IEMBS.2005.1615654
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-01-17
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Cells (biology) Amino acids Bones Stem cells In vitro Cloning Scanning electron microscopy Testing Immune system Neutron spin echo
Content Type	Text
Resource Type	Article

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