NDLI: BAEC adhesion analysis using Thickness Shear Mode sensor

Content Provider	IEEE Xplore Digital Library
Author	Hong, S. Ergezen, E. Barbee, K.A. Lec, R.
Copyright Year	2005
Description	Author affiliation: Sch. of Biomed. Eng., Sci. & Health Syst., Drexel Univ., Philadelphia, PA (Hong, S.; Ergezen, E.; Barbee, K.A.; Lec, R.)
Abstract	A thickness shear mode (TSM) sensor of which acoustic shear wave has a nano-scale penetration depth was used in detecting biological activities of living cells. The initial cell adhesion process was characterized by analyzing multi-harmonic responses of the TSM sensor. A suspension of bovine aortic endothelial cells (BAECs) was placed on the TSM sensor, and changes in impedance magnitude during sedimentation and attachment processes were monitored. For the validation of cell adhesion, the sensor surface was rinsed at various time points during the adhesion process, and the number of cells remaining attached was counted. By comparing sensor signals with the independently measured cell adhesion, sensor readings were correlated with physical adhesion process. In addition, by coating the TSM sensors with gelatin and by blocking the integrin bindings with Arg-Gly-Asp peptides (RGD), we investigated cell adhesion process under different environments. The time course of sensor readings were characterized with delay time, slope and maximum value of impedance magnitude changes. This study demonstrates the ability of the TSM sensor to detect distinct phases in the cell adhesion process
Starting Page	1047
Ending Page	1050
File Size	299093
Page Count	4
File Format	PDF
ISBN	0780387414
DOI	10.1109/IEMBS.2005.1616598
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-01-17
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Adhesives Sensor phenomena and characterization Biosensors Acoustic sensors Acoustic waves Nanobioscience Acoustic signal detection Cells (biology) Bovine Surface impedance
Content Type	Text
Resource Type	Article

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