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| Content Provider | Scientific Electronic Library Online (SciELO) |
|---|---|
| Author | Zhao, Yang Wang, Qian Zang, Bin |
| Abstract | Abstract Purpose: To explore the effect of milk fat globule-epidermal growth factor 8 (MFG-E8) on sepsis-induced acute kidney injury (SAKI). Methods: Male C57BL/6 mice were randomized to control, sham, CLP, CLP+PBS, and CLP+rmMFG-E8 groups. SAKI was induced by cecal ligation and puncture (CLP). Recombinant mouse MFG-E8 (rmMFG-E8) (20 μg/kg) or PBS (vehicle) was administered intraperitoneally. Blood, urine and renal tissue were collected at 24 h after CLP. Blood samples were tested for serum kidney injury biomarker and cytokines. Urine samples were collected to detect KIM-1, and NGAL. Real-time PCR was tested for Bax and Bcl-2. TUNEL staining was used to determine renal apoptosis. Western blot was used to detect the expression of Bax, Bcl-2, and proteins in the NF-κB pathway. Results: MFG-E8 alleviated SAKI by decreasing serum Cre, BUN, urine KIM-1 and NGAL and by mitigating renal pathological changes significant (p < 0.05). IL-1β, IL-6, TNF-α were significantly inhibited by MFG-E8 (p < 0.05). Apoptosis induced by SAKI was markedly suppressed by MFG-E8. Finally, MFG-E8 attenuated the activation of the NF-��B signaling pathway in SAKI. Conclusion: MFG-E8 has beneficial effects on SAKI, which may be achieved by inhibiting the NF-κB pathway. |
| Related Links | http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-86502019000200208&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019000200208&lng=en&nrm=iso |
| File Format | |
| ISSN | 01028650 |
| DOI | 10.1590/s0102-8650201900209 |
| Journal | Acta Cirurgica Brasileira |
| Issue Number | 2 |
| Volume Number | 34 |
| Language | English |
| Publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| Publisher Date | 2019-01-01 |
| Access Restriction | Open |
| Subject Keyword | Acute Kidney Injury Apoptosis Inflammation NF-kappa B Mice |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surgery |
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