NDLI: Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold

Content Provider	Scientific Electronic Library Online (SciELO)
Author	Bertanha, Matheus Sobreira, Marcone Lima Bovolato, Ana Lívia de Carvalho Rinaldi, Jaqueline de Carvalho Reis, Patricia Pintor Moroz, Andrei Moraes, Leonardo Nazario de Deffune, Elenice
Abstract	Abstract Purpose: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. Methods: Three groups were created for ultrastructural and residual DNA analysis: group 1 - control, consisting of samples of vena cava in natura; group 2 - SD, consisting of vein fragments submitted to 2% sodium deoxycholate decellularization by shaking (160rpm - Shaker News Brunswick Scientific®) for 1 hour at controlled temperature shaker at 37°C; group 3 - SDS, consisting of vein fragments submitted to 1% sodium dodecyl sulfate decellularization under the same previous condition, for 2 hours. Results: The ultrastructural matrix of the blood vessel maintained its vintegrity after either decellularization models. The results of the two quantification methods demonstrated a significant decrease in the DNA content of the decellularized vena cava samples as compared to the control samples and, differed statistically from each other, p <0.05. Conclusion: The 2% DS protocol for vein decellularization, in this experimental model, was considered the best protocol because it presented less amount of residual DNA without causing substantial destruction of the extracellular matrix.
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-86502017000900706&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000900706&lng=en&nrm=iso
Starting Page	706
Ending Page	711
Page Count	6
File Format	PDF
ISSN	01028650
DOI	10.1590/s0102-865020170090000003
Journal	Acta Cirurgica Brasileira
Issue Number	9
Volume Number	32
Language	English
Publisher	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Publisher Date	2017-09-01
Access Restriction	Open
Subject Keyword	Tissue Scaffolds Peripheral Arterial Disease Blood Vessels Tissue Engineering Rabbits
Content Type	Text
Resource Type	Article
Subject	Surgery

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