NDLI: Corneal angiogenesis based on different protocols of alkaline cauterization in murine models

Content Provider	Scientific Electronic Library Online (SciELO)
Author	Claros-Chacaltana, Flor Diana Yokoay Kobashigawa, Karina Kamachi Padua, Ivan Ricardo Martinez Valdetaro, Gisele Pereira Aldrovani, Marcela Laus, José Luiz
Abstract	Abstract Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-86502017000800607&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000800607&lng=en&nrm=iso
Starting Page	607
Ending Page	616
Page Count	10
File Format	PDF
ISSN	01028650
DOI	10.1590/s0102-865020170080000002
Journal	Acta Cirurgica Brasileira
Issue Number	8
Volume Number	32
Language	English
Publisher	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Publisher Date	2017-08-01
Access Restriction	Open
Subject Keyword	Corneal Neovascularization Cautery Silver Rats.
Content Type	Text
Resource Type	Article
Subject	Surgery

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