NDLI: Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice

Content Provider	Scientific Electronic Library Online (SciELO)
Author	Lee, Edwin Jin Su Silva, Sonia Maria da Simões, Manuel de Jesus Montero, Edna Frasson de Souza
Abstract	PURPOSE: Evaluate the effect of N-acetylcysteine in liver remnant after hepatectomy associated to ischemia-reperfusion injury in mice. METHODS: Male adult BALB/c mice, weighing 20-22g were used. Animals were anesthetized with ketamine (70 mg/kg) and xylazine (10 mg/kg); received N-acetylcysteine (150 mg/kg, H-IR-NAC group) or vehicle (H-IR group). Surgical procedures were performed under 10X magnification. Partial hepatectomy (30%) was followed by ischemia-reperfusion injury (30 minutes of ischemia and 60 minutes of reperfusion). Blood sample and liver tissue were removed before animal was euthanized. AST and ALT were evaluated in blood samples and histomorphological analyses were performed in remnant liver. Groups were compared by Mann-Whitney test, and it was considered significant when p<0.05. RESULTS: Biochemical evaluations showed reduced levels of ALT in NAC group (H-IR-NAC=376±127U/l vs H-IR=636±39U/l, p=0.023). AST was similar (p=0.456). H-IR group showed hepatic tissue with preserved architecture, large area of steatosis, vascular congestion and rare mitogenic activity. NAC group showed hepatic tissue with small area of steatosis, vascular congestion and elevated mitogenic activity, evidenced by increased binuclear cells (H-IR-NAC=15.88±0.52 vs H-IR=7.4±0.37, p<0.001). CONCLUSION: N-acetylcysteine promotes enzymatic and morphological protection against hepatectomy and ischemia-reperfusion injury.
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-86502012000400011&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000400011&lng=en&nrm=iso
Starting Page	346
Ending Page	349
Page Count	4
File Format	PDF
ISSN	01028650
DOI	10.1590/S0102-86502012000400011
Journal	Acta Cirurgica Brasileira
Issue Number	4
Volume Number	27
Language	English
Publisher	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Publisher Date	2012-04-01
Publisher Institution	UNIFESP Division of Operative Technique and Experimental Surgery Department of Surgery UNIFESP Department of Surgery UNIFESP Division of Histology Department of Morphology
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Acetylcysteine Reperfusion Injury Hepatectomy Mice Acetilcisteína Traumatismo por Reperfusão Hepatectomia Camundongos
Alternative Title	Efeito da N-acetilcisteína na lesão hepática por isquemia e reperfusão após 30% de hepatectomia em camundongos
Content Type	Text
Resource Type	Article
Subject	Surgery

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