NDLI: Preconditioning with L-alanyl-glutamine reduces hepatic ischemia-reperfusion injury in rats

Content Provider	Scientific Electronic Library Online (SciELO)
Author	Araújo Júnior, Raimundo José Cunha Silva Júnior, Raimundo Gerônimo da Vasconcelos, Marcelo Pinho Pessoa de Guimarães, Sérgio Botelho Vasconcelos, Paulo Roberto Leitão de Garcia, José Huygens Parente
Abstract	PURPOSE: To evaluate the effects of pre-conditioning with L-alanyl- glutamine (L-Ala-Gln) in rats subjected to total hepatic ischemia. METHODS: Thirty Wistar rats, average weight 300g, were randomly assigned to 3 groups (n=10): G-1 - Saline, G-2- L-Ala-Gln, G-3-control (Sham). G-1 and G-3 groups were treated with saline 2.0 ml or L-Ala-Gln (0.75mg/Kg) intraperitoneally (ip) respectively, 2 hours before laparotomy. Anesthetized rats were subjected to laparotomy and total hepatic ischemia (30 minutes) induced by by clamping of portal triad. Control group underwent peritoneal puncture, two hours before the sham operation (laparotomy only). At the end of ischemia (G1 and G2), the liver was reperfused for 60 minutes. Following reperfusion blood samples were collected for evaluation of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels. Liver (medium lobe) was removed for immunohistochemistry study with antibody for Caspase-3. RESULTS: It was found a significant decrease (p<0.05) of ALT levels (270.6 +40.8 vs 83.3 +5.5 - p <0.05), LDH (2079.0 +262.4 vs. 206.6 +16.2 - p <0.05) and Caspase-3 expression (6.72 +1.35 vs. 2.19 +1.14, p <0.05) in rats subjected to I / R, comparing the group treated with L-Ala -Gln with G-2. Also, the ALT level was significantly lower (P<0.05) in G-1 and G-2 groups than in G-3 (control group). CONCLUSION: L-Ala-Gln preconditioning in rats submitted to hepatic I/R significantly reduces ALT, LDH and Caspase-3 expression, suggesting hepatic protection.
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-86502011000700003&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000700003&lng=en&nrm=iso
Starting Page	8
Ending Page	13
Page Count	6
File Format	PDF
ISSN	01028650
DOI	10.1590/S0102-86502011000700003
Journal	Acta Cirurgica Brasileira
Volume Number	26
Language	English
Publisher	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Publisher Date	2011-01-01
Publisher Institution	UFC Department of Surgery Surgical Pathology and Cytology Laboratory UFC Faculty of Medicine
Publisher Place	Brazil Brazi
Access Restriction	Open
Subject Keyword	Surgical Procedures Ischemia Reperfusion Glutamine Liver Rats Procedimentos Cirúrgicos Operatórios Isquemia Reperfusão Glutamina Fígado Ratos
Alternative Title	Pré-condicionamento com L-alanil-glutamina reduz a lesão de isquemia-reperfusão no fígado de ratos
Content Type	Text
Resource Type	Article
Subject	Surgery

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