NDLI: Effects of platelet-rich plasma gel on skin healing in surgical wound in horses

Content Provider	Scientific Electronic Library Online (SciELO)
Author	DeRossi, Rafael Coelho, Anna Carolina Anciliero de Oliveira Mello, Gisele Silveira de Frazílio, Fabrício Oliveira Leal, Cássia Rejane Brito Facco, Gilberto Gonçalves Brum, Karine Bonucielli
Abstract	PURPOSE: To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine. METHODS: To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair. RESULTS: The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation. CONCLUSION: Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equine
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-86502009000400006&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000400006&lng=en&nrm=iso
Starting Page	276
Ending Page	281
Page Count	6
File Format	PDF
ISSN	01028650
DOI	10.1590/S0102-86502009000400006
Journal	Acta Cirurgica Brasileira
Issue Number	4
Volume Number	24
Language	English
Publisher	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Publisher Date	2009-08-01
Publisher Institution	UFMS Faculty of Veterinary Medicine and Animal Science Department of Veterinary Medicine, Surgery and Anesthesiology University for Development of the State and the Pantanal Region Division of Surgery UFMS Post-graduate in Health and Development, West Central Region UNIDERP Division of Pathology UFMS Department of Pathology
Publisher Place	Brazil Brazi
Access Restriction	Open
Subject Keyword	Platelet-Rich Plasma Wound Healing Skin Horses Plasma Rico em Plaquetas Cicatrização de Feridas Pele Cavalos
Alternative Title	Efeitos do gel de plasma rico em plaquetas na cicatrização de feridas cirúrgicas na pele de cavalos
Content Type	Text
Resource Type	Article
Subject	Surgery

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