NDLI: Reduction of podocytes number in late diabetic alloxan nephropathy: prevention by glycemic control

Content Provider	Scientific Electronic Library Online (SciELO)
Author	Macedo, Célia Sperandéo Lerco, Mauro Masson Capelletti, Sônia Maria Silva, Reinaldo José Pinheiro, Daniela de Oliveira Spadella, César Tadeu
Abstract	PURPOSE: To determine podocyte number and GBM thickness in diabetic rats either under glycemic control or without glycemic control at 6 and 12 months after diabetes induction. METHODS: 100 wistar rats weighing 200-300g were divided into 6 groups: Normal group (N6 and N12- 25 rats); Diabetic group (D6 and D12- 25 rats), diabetic treated group ( DT 6 and DT 12- 25 rats) on insulin 1,8- 3,0 IU/Kg associated with acarbose (50mg to 100g of food) daily mixed in chow. Alloxan was injected intravenously in a dose of 42 mg/Kg of weight. Body weight, waterintake, 24-h diuresis, glycemia and glucosuria were determined before induction, 7 and 14 days after induction and monthly thereafter. Treatment started at day 14. Three groups were sacrificed at 6 months (N6,D6, DT6) and 3 groups at 12 months (N12, D12, DT12) with the renal tissue being prepared for electron microscopy. RESULTS: Glycemia in DT6¨and in DT12 was significantly different from that in D6 and D12 rats and similar to that in N6 and N12 animals. The number of podocytes in DT6 was not different from that in N6 and D6 (median = 11); the number of podocytes in DT12 (median = 11) differed from that in D12 (median = 8), but not from that in N12 (median = 11). GBM thickness in D6 (0.18 micrometers) was lower than in D12 (0.29 micrometers); while in DT6 (0.16 micrometers) it was lower than in D6 (0.18 micrometers). In DT12 (0.26 micrometers), it was lower than in D12 (0.29 micrometers). CONCLUSION: The control of hyperglycemia prevented GBM thickening in early and late (12 mo) alloxan diabetic nephropathy and podocyte number reduction.
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-86502007000500003&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000500003&lng=en&nrm=iso
Starting Page	337
Ending Page	341
Page Count	5
File Format	PDF
ISSN	01028650
DOI	10.1590/S0102-86502007000500003
Journal	Acta Cirurgica Brasileira
Issue Number	5
Volume Number	22
Language	English
Publisher	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Publisher Date	2007-10-01
Publisher Institution	UNESP School of Medicine Department of Pediatrics UNESP School of Medicine Department of Surgery and Orthopedics UNESP Institute of Biosciences Department of Morphology UNESP Institute of Biosciences Department of Parasitology
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Diabetes Mellitus Experimental Microscopy Electron Podocytes Rats Diabetes Mellitus Experimental Microscopia Eletrônica Podócitos Ratos
Alternative Title	Redução do número de podócitos na fase tardia da nefropatia diabética aloxânica: prevenção pelo controle glicêmico
Content Type	Text
Resource Type	Article
Subject	Surgery

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