NDLI: Comparison of Defense Related Enzyme between Oligochitosan Induced Protein Kinase Gene Silenced Transgenic Tobacco and Wild Type Tobacco

Content Provider	IEEE Xplore Digital Library
Author	Chen Yafei Zhan Yong Guo Peng Liu Hui Han Yingrong Zhang Yuhong Gao Liya Zhao Xiaoming Du Yuguang
Copyright Year	2008
Description	Author affiliation: Sch. of Sci., Hebei Univ. of Technol., Tianjin (Chen Yafei; Zhan Yong; Guo Peng; Liu Hui; Han Yingrong; Zhang Yuhong; Gao Liya)
Abstract	Oligochitosan (OC) could regulate plant defense responses in many aspects, but the basis signal transduction pathway is still unclear. In this study, we use transgenic (TG) tobacco as plant material whose OIPK gene was inhibited by antisense transformation, to identify the role oligochitosan induced protein kinase (OIPK) played in OC treated tobacco plants. Pathogen-related (PR) proteins enzymes activity assays and reverse transcriptional polymerase (RT-PCR) were conducted to compare the differences between TG and the corresponding wild type (WT) tobacco plants. Firstly, it was validated that OIPK could induce Phenylalanine ammonialyase (PAL, E.C. 4.3.1.5) and peroxidase (POD, E.C. 1.11.1.7 ) activities in respect to both activation time and activation fold after OC treatment, while OIPK had no influences on polyphenol oxidase (PPO, E.C.I.14.18.1) activity. Secondly, it was found that in TG tobacco, there was nearly just basis pal transcriptional expression after OC treatment, which showed that OIPK could determine pal gene expression in some sense. Thirdly, OIPK could enhance defense related gene transcription chi and glu activation intension and rapidity.
Starting Page	1076
Ending Page	1079
File Size	116841
Page Count	4
File Format	PDF
ISBN	9781424417476
DOI	10.1109/ICBBE.2008.263
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Fungi Pathogens Materials science and technology Microorganisms Amino acids Biochemistry Polymers Gene expression Conducting materials
Content Type	Text
Resource Type	Article

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