NDLI: Study of Transformation and Chromosomal Instability of Human Tracheal Epithelial Cells Induced by Formaldehyde

Content Provider	IEEE Xplore Digital Library
Author	Zhang Gui-zhi Chen Li Xiong Wei Zhang Le-feng Li Bian-lan Liu Chang-ting Liu Dong-shan
Copyright Year	2008
Description	Author affiliation: Gen. Hosp. of People Liberation Army, Beijing (Zhang Gui-zhi)
Abstract	Objective: To determine the mechanisms of chemical carcinogenesis, the relationship between malignant transformation and genome /chromosomal instability of human bronchial epithelial cell, cell line - BEAS-2B cell exposed to formaldehyde was studied. Methods: The $LC_{50}$ value of formaldehyde to BEAS-2B cells was tested by MTT assay. Then the cells were induced with formaldehyde at the concentration of 20% $LC_{50};$ the characteristics of transformation of cells were identified though the characters of growth curve and colony formation rate of semi-solid agar. The metaphase modal chromosome numbers, karyotypes and nonrandom structural change of induced cell strains were analysed with the G-banding technique. Results: The growth curve of induced cells showed a trend of infinite proliferation and the anchorage independent growth was appeared in 15 generation cells. Before transformed, BEAS-2B cells had a pseudodiploid karyotype and were relatively stable genotypically. After transformed, three characteristic abnormal chromosomes were found. Most of the induced cells were triploid and/or tetraploid cells. The loss of one normal or two homologs of chromosome 14 also had a higher frequency. Furthermore, in their karyotypes, one or two unknown chromosomes appeared and trisomy-5 or trisomy-16 was observed. The induced cells also had a higher frequency of non-stabilizing aberration (39%), including chromosome loss, endoduplication, translocation, break, dicentromere and/or tricentromere etc. Conclusion: Formaldehyde could contribute to progression of transformation and result in genome instability.
Starting Page	3934
Ending Page	3937
File Size	206945
Page Count	4
File Format	PDF
ISBN	9781424417476
DOI	10.1109/ICBBE.2008.485
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Humans Genomics Cells (biology) Frequency Capacitive sensors Bioinformatics Biological cells Chemicals Cancer Testing
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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