NDLI: Anti- Esophageal Cancer Active Immunity Induced by FasL/B7-1 Genes Modified Tumor Cells

Content Provider	IEEE Xplore Digital Library
Author	Shi-Ying Zheng Jun Zhao Jin-Feng Ge Hong Li
Copyright Year	2008
Description	Author affiliation: Dept. of Cardio-Thoracic Surg., First Affiliated Hosp. of Suzhou Univ., Suzhou (Shi-Ying Zheng; Jun Zhao; Jin-Feng Ge)
Abstract	To study the activation of CTLs against esophageal cancer cells induced by FasL/B7-1 (FB-11)genes modified tumor cells, and to explore whether co-expression of FasL and B7-1 in Eca-109 tumor cells could initiate an synergistic antitumor effect. FasL and B7-1 genes were transfected into human Eca-109 Eca-109 cancer cells with adenovirus vectors. The positive clones were selected by G418. FasL and B7-1 were detected by Flow cytometry and RT-PCR . The abdominal infiltrating lymphocytes and sensitized spleen cells were obtained from the mice who were immunized with Eca-109/FB-11 or wild type Eca-109 cells intraperitoneally, and the cytotoxicity of these CTLs against tumor cells was determined by MTT assay. Flow cytometry and RT-PCR showed that FasL and B7-1 were highly expressed. $FasL^{+}/B7-1^{+}$ Eca-109 cells (Eca-109/FB-11) were inoculated subcutaneously in the dorsal skin of C57BL/6 mice and then they decreased their tumorigenicity greatly (z=2.15-46.10, p<0.01). The Eca-109/FB-11 cell-sensitized mice obtained the protective immune activity against the rechallenge of wild type Eca-109 cells (z=2.06-44.30, p<0.05). It was showed that the cytotoxicity of CTLs induced by Eca-109/FB-11 cells against Eca-109 was significantly higher than that of CTLs activated by wild-type Eca-109 cells (84.1plusmn2.4% vs 30.5plusmn2.3%, p<0.05). The results suggest that the FasL and B7-1 can effectively promote the activity of CTLs against esophageal cancer cells.
Starting Page	407
Ending Page	414
File Size	353470
Page Count	8
File Format	PDF
ISBN	9781424417476
DOI	10.1109/ICBBE.2008.103
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Humans Cloning Mice Skin Protection Esophagus Cancer Tumors Immune system Abdomen
Content Type	Text
Resource Type	Article

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