NDLI: Analysis of stress-induced or salicylic acid-induced expression of the pathogenesis-related 1a protein gene in transgenic tobacco.

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Analysis of stress-induced or salicylic acid-induced expression of the pathogenesis-related 1a protein gene in transgenic tobacco.

Content Provider	Semantic Scholar
Author	Ohshima, Masahiro Itoh, Hiroaki Matsuoka, Makoto Murakami, Tadayuki Ohashi, Yohei
Copyright Year	1990
Abstract	The cis-acting elements for regulating gene expression of the tobacco pathogenesis-related 1a protein gene were analyzed in transgenic plants. The 5'-flanking 2.4-kilobase fragment from the pathogenesis-related 1a protein gene was joined to the bacterial beta-glucuronidase gene and introduced into tobacco cells by Agrobacterium-mediated gene transfer. Promoter activity was monitored by quantitative and histochemical assay of beta-glucuronidase activity in leaves of regenerated transgenic plants. The level of beta-glucuronidase activity was clearly increased by treatment with salicylic acid, by cutting stress, and by local lesion formation caused by tobacco mosaic virus infection. Cytochemical studies of the induced beta-glucuronidase activity revealed tissue-specific and developmentally regulated expression of the pathogenesis-related 1a gene after stress or chemical treatment and after pathogen attack. To identify the cis-acting element more precisely, a series of 5'-deleted chimeric genes was constructed and transformed into tobacco plants. Transgenic plants with a 0.3-kilobase fragment of the 5'-flanking region of the pathogenesis-related 1a gene had the same qualitative response as those with the 2.4-kilobase fragment upon treatment with salicylic acid or infection with TMV. Thus, the 0.3-kilobase DNA sequence fragment was sufficient to allow the regulated expression of the pathogenesis-related 1a gene.
Starting Page	95
Ending Page	106
Page Count	12
File Format	PDF HTM / HTML
DOI	10.1105/tpc.2.2.95
PubMed reference number	2136635
Journal	Medline
Volume Number	2
Issue Number	2
Alternate Webpage(s)	http://www.plantcell.org/content/plantcell/2/2/95.full.pdf
Alternate Webpage(s)	https://doi.org/10.1105/tpc.2.2.95
Journal	The Plant cell
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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Analysis of Stress-Induced or Salicylic Acid-Induced Expression of the Pathogenesis-Related 1a Protein Gene in Transgenic Tobacco

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