NDLI: 2D DIGE Data Analysis for Differential Protein Spot Identification

Content Provider	IEEE Xplore Digital Library
Author	Lihua Li Lei Zhu Bin Han Li Chen Sutphen, R.
Copyright Year	2008
Description	Author affiliation: Inst. for Biomed. Eng. & Instrum., Hangzhou Dianzi Univ., Hangzhou (Lihua Li; Lei Zhu; Bin Han)
Abstract	Differential in-gel electrophoresis (DIGE) technique has been used for differential protein analysis to improve the reproducibility of comparative 2D gel experiments. Because the sample size in 2D DIGE experiments is usually very small, the traditional statistical methods such as student t-test could not provide an accurate and reliable detection/identification of differentially expressed protein spots. This paper proposed a ranking based approach for DE protein spot identification, in which the information of comparative ranks of the protein spot volume ratio rather than their distribution were used in selection. Two methods were designed to integrate the ranking information from a set of pair-wise comparisons. The evaluation results demonstrated that the proposed methods are effective in DE protein spot identification when sample size is small.
Starting Page	620
Ending Page	623
File Size	228942
Page Count	4
File Format	PDF
ISBN	9781424417476
DOI	10.1109/ICBBE.2008.151
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Protein engineering Data analysis Instruments Electrokinetics Mass spectroscopy Reproducibility of results Statistics Pixel Cancer Biomedical engineering
Content Type	Text
Resource Type	Article

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