NDLI: Microbial Diversity Research of Produced Water from Daqing Oil Reservoir after Polymer Flooding

Content Provider	IEEE Xplore Digital Library
Author	She Yuehui Zhang Xuemei Zhang Fan Wang Zhengliang Shu Fuchang Zhou Lingge Xiang Tingsheng Yu Longjiang
Copyright Year	2008
Description	Author affiliation: Coll. of Life Sci. & Technol., Huazhong Univ. of Sci. & Technol., Wuhan (She Yuehui)
Abstract	DNA was extracted from four oil well samples in Daqing oil reservoir after polymer flooding. A 16S rDNA clone library was constructed by PCR amplification applying universal primers, cloned in E.coli and subsequently characterized by amplified rDNA restriction analysis (ARDRA). 298 clones were obtained and classified into 22 different operational taxonomic units (OTUs). Four OTUs occupied 78.18 percent of the 16S rDNA clone library. Nine OTUs have only one clone. At the same time, the microbial diversity of the 16S rDNA clone library was evaluated by coverage value (C), Shannon-Weiner index (H) and Evenness index (E). Phylogenetic analysis showed that the dominant members of the library were affiliated with the Alphaproteobacteria (20.47%), the gammaproteobacteria (23.81%), the Deltaproteobacteria (32.21%) and the uncultured bacteria (21.16%). Pseudomonas and Acinetobacter which have the connection with biodegradation of hydrocarbon and production of surfactant occupied 17.79% and 6.02% in the clone library, respectively.
Starting Page	771
Ending Page	775
File Size	295501
Page Count	5
File Format	PDF
ISBN	9781424417476
DOI	10.1109/ICBBE.2008.188
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microorganisms Cloning DNA Libraries Phylogeny Polymers Floods Hydrocarbon reservoirs Water resources Petroleum
Content Type	Text
Resource Type	Article

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