NDLI: The Effect of Freeze Drying on the Deoxyribonucleic Acid Chain of Human Sperm

Content Provider	IEEE Xplore Digital Library
Author	Wang Peitao Shao Cuihua Shen Daqing Li Qiang Liu Zhongqiang Fu Xiuyan Li Huaqin
Copyright Year	2008
Description	Author affiliation: Dept. of Cryomedicine, Affiliated Hosp. of Qingdao Univ. Med. Sch., Qingdao (Wang Peitao; Shao Cuihua; Shen Daqing; Li Qiang; Liu Zhongqiang; Fu Xiuyan; Li Huaqin)
Abstract	As a new method for preservating human spermatozoa, freeze drying has many special advantages. However, the freeze drying process could damage the the DNA of the human spermatozoa, the injury will also be caused by the endoenzyme of the spermatozoa. So far, the commonly used freeze drying protective agents-ETBS (ethylenedioxy-diethylene- dinitrilo-tetraacetic acid (EDTA) or ethylene glycol-bis-(2- aminoethyl)-N,N,N', N'-tetraacetic acid (EGTA) was added to the Tris-HCl buffer) has been proved to restrain the enzyme activation in sperm and protect the genetic material. Many studies showed that yolk and trehalose can supply special protection for the sperm in the freeze drying process. In this study, terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) and single cell gel electrophoresis (SCGE) were used to detect the sperm DNA damage after freeze drying with ETBS, ETBS added with trehalose or added with both trehalose and yolk as protective agents.
Starting Page	919
Ending Page	923
File Size	133064
Page Count	5
File Format	PDF
ISBN	9781424417476
DOI	10.1109/ICBBE.2008.226
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Hospitals Animals Instruments Humans DNA Genetics Biochemistry Nitrogen Protection Injuries
Content Type	Text
Resource Type	Article

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