NDLI: Relationship between Caspase-3 Expression and Apoptosis in Lung Adenocarcinoma Cell A-549 with Adenovirus-Mediated FasL Gene

Content Provider	IEEE Xplore Digital Library
Author	Shi-ying Zheng Hong Li Dong Jiang Jin-feng Ge
Copyright Year	2008
Description	Author affiliation: Dept. of Thoracocardiac Surg., First Affiliated Hosp. of Suzhou Univ., Suzhou (Shi-ying Zheng)
Abstract	Investigate the relationship between caspase-3 expression and apoptosis in lung adenocarcinoma cell line A-549 who was transfected with Adenovirus-mediated FasL gene. Human lung adenocarcinoma cell line A-549 was transfected with Adenovirus-mediated FasL gene. The expression of caspase-3-, apoptosis morphological changes were measured using flow cytometry, RT-PCR assays, the activity of caspase-3 and cleavage of poly ADP-ribose polymerase (PARP) were determined with Western blot analysis. The expression of caspase-3 was low in lung adenocarcinoma cell line A-549, but transfection of lung adenocarcinoma cells with Ad-FasL enhanced cell expression of caspase-3 gene and induced susceptibility to Fas/FasL mediated apoptosis(44.08% vs 18.15%, p<0.01). Apoptosis had positive relationship with the activity of caspase-3 ( gamma =0.647, p<0.01). Down-regulation of caspase-3 expression and resistance to Fas/FasL-mediated apoptosis in lung adenocarcinoma cells were found to be related to the development and progression of lung adenocarcinoma cells. FasL gene transfer may increase caspase-3 in lung adenocarcinoma cells and enhance their apoptosis.
Starting Page	423
Ending Page	428
File Size	212124
Page Count	6
File Format	PDF
ISBN	9781424417476
DOI	10.1109/ICBBE.2008.105
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Hospitals Lungs Humans Birth disorders Biomembranes Biochemistry Manufacturing Immune system Cancer
Content Type	Text
Resource Type	Article

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