NDLI: Repression of Nrf1-Mediated Transactivation by MCRS2

Content Provider	IEEE Xplore Digital Library
Author	Wu, J.-L. Wu, S.-F. Lin, Y.-S. Lin, Y.-J. Lin, Y.-T. Huang, C.-F.
Copyright Year	2008
Description	Author affiliation: IBMS Acad. Sinica Taipei, Taipei (Wu, J.-L.)
Abstract	Nrfl [p45 nuclear factor-erythroid 2 (p45 NF-E2)- related factor 1], a member of the CNC-bZIP (CNC basic region leucine zipper) family, is known to be a transcriptional activator via dimerization with distinct partners, such as Maf, FosB, c-Jun, JunD, etc. To find other proteins interacting with the CNC bZIP domain, the CNC-bZIP region of Nrfl was used as a bait in a yeast two-hybrid screening. MCRS2, a splicing variant of p78/MCRSl, was isolated as the Nrfl- interacting partner. The interaction between Nrfl and MCRS2 was confirmed in vitro by GST pull-down assays and in vivo by co-immunoprecipitation. The Nrfl-MCRS2 interaction domains were mapped to be the residues 354-447 of Nrfl and the residues 314-475 of MCRS2 respectively by yeast two- hybrid and GST pull- down assays. To further confirm the biological relevance, a reporter driven by CNC-bZIP protein binding sites was also shown to be repressed by MCRS2 in transient transfection assay. And a reporter gene activated by LexA-Nrfl was specifically repressed by MCRS2. Taken together, these results pointed to a new Nrfl-interacting protein MCRS2 that can mediate repression effect on Nrfl- mediated transcriptional activation. Until now, this is the first repressor protein on Nrfl transactivation activity and there is no other repressor (s) reported before.
Starting Page	391
Ending Page	394
File Size	241136
Page Count	4
File Format	PDF
ISBN	9781424417476
DOI	10.1109/ICBBE.2008.99
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Fungi Biotechnology Sequences Technology management Humans Amino acids Educational institutions Gene expression Computer numerical control
Content Type	Text
Resource Type	Article

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