NDLI: The electric slide: dielectrophoresis for high precision cell patterning

Content Provider	IEEE Xplore Digital Library
Author	Gray, D.S. Chen, C.S.
Copyright Year	2002
Description	Author affiliation: Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA (Gray, D.S.; Chen, C.S.)
Abstract	Arrays of precisely positioned cells have many potential applications in biotechnology and basic cell biology. We have created arrays of single cells using dielectrophoresis (DEP), the force on polarizable bodies in a non-uniform electric field. Charge buildup across the plasma membrane was minimized by the application of voltages with high frequency and low amplitude. Cells cultured under these conditions were similar in gross morphology and growth rate to tissue culture controls. With DEP-based trapping, we have patterned multiple cell layers and multiple types of cells on chemically uniform substrates. We have also used DEP patterning to position cells on surfaces patterned with cell-adhesive areas surrounded by non-adhesive regions. This dual approach allowed us to organize cells in patterns that were different from the adhesive pattern. Using cells patterned by DEP, we investigated the effects of heterotypic, cell-cell interactions and cell packing on proliferation and other cellular processes. In the future, DEP will facilitate the construction of cell-based biosensors, functional nerve chips, and large cellular arrays for high throughput screening.
Starting Page	1686
Ending Page	1687
File Size	187086
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1106602
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Dielectrophoresis Cells (biology) Biotechnology Biological cells Polarization Nonuniform electric fields Plasma applications Plasma chemistry Biomembranes Voltage
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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