NDLI: Nanoengineered fluorescent sensors containing enzyme assays

Content Provider	IEEE Xplore Digital Library
Author	Nayak, S.R. Guice, K. Lvov, Y. McShane, M.J.
Copyright Year	2002
Description	Author affiliation: Inst. for Micromanufacturing, Louisiana Tech. Univ., Ruston, LA, USA (Nayak, S.R.; Guice, K.; Lvov, Y.; McShane, M.J.)
Abstract	Describes progress towards the fabrication, calibration and testing of polyelectrolyte microshells being used as sensors for biochemicals, which are either produced or consumed in enzyme-catalyzed reactions. They are nanoengineered using layer-by-layer (LbL) self-assembly. These shells are loaded with the analyte sensitive fluorophore, the reference fluorophore for ratiometric monitoring and the enzyme, which catalyzes the reaction. A potential use of these sensors is to study the transient local changes in both glucose and lactate caused by neuronal activity. A specific application of this project is the study of the brain's response to low glucose. This paper primarily focuses on the work done on enzyme encapsulation. A study of the effect of encapsulation on enzymes as well as the influence of sensor fabrication variables (size, layer thickness, composition) on the response characteristics of the sensors is being investigated. Preliminary work has shown that the enzymes can be encapsulated by a method of aided diffusion and that the enzyme retains its activity after encapsulation. Co-loading of the labeled enzyme and the oxygen sensitive dye has also been demonstrated.
Starting Page	1679
Ending Page	1680
File Size	137846
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1106599
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence Biochemistry Biosensors Sensor phenomena and characterization Encapsulation Fabrication Sugar Calibration Testing Self-assembly
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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