NDLI: Micro-electric impedance spectra of isolated cells recorded in micro-channels

Content Provider	IEEE Xplore Digital Library
Author	Ayliffe, H.E. Brown, S.D. Rabbitt, R.D.
Copyright Year	2002
Description	Author affiliation: Dept. of Bioeng., Utah Univ., Salt Lake City, UT, USA (Ayliffe, H.E.; Brown, S.D.; Rabbitt, R.D.)
Abstract	Electric impedance (EI) spectra (1kHz-20Mhz) of isolated polymorphonuclear leukocytes and red blood cells were recorded by positioning individual cells between pairs of metal electrodes in slender rectangular channels (/spl sim/40 10/sup -12/ m/sup 2/ cross-sectional area). The present study was designed to demonstrate that cell type, viability and dielectric properties can be discerned on the basis of raw micro-EI spectral data. Each micro-EI MEMS device was tested to quantify the frequency-dependent impedance of the electrode-electrolyte interface at the experimental current density. Sensitivity of the technique to changes in sample properties was tested by filling the recording zone with a variety of materials and cells with known dielectric properties. Small changes in cell media ionic concentration and cellular dielectric properties were quantifiable by analysis of EI data. Results further show that micro-EI signatures of the device when loaded with red blood cells (RBCs) vs. polymorphonuclear leukocytes (PMNs), or vital vs. non-vital PMNs were significantly different thus allowing cell identification on the basis of spectral signatures.
Starting Page	1692
Ending Page	1693
File Size	206924
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1106605
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Impedance Dielectrics White blood cells Red blood cells Cells (biology) Electrodes Microelectromechanical devices Testing Frequency Current density
Content Type	Text
Resource Type	Article

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