NDLI: Micro-patterning of nanosensor elements using layer-by-layer self-assembly, avidin-biotin chemistry, and photolithography

Content Provider	IEEE Xplore Digital Library
Author	Kondabatni, K.K. Hua, F. Cui, T. Lvov, Y. McShane, M.J.
Copyright Year	2002
Description	Author affiliation: Inst. for Micromanufacturing, Louisiana Tech. Univ., Ruston, LA, USA (Kondabatni, K.K.; Hua, F.; Cui, T.; Lvov, Y.; McShane, M.J.)
Abstract	The miniaturization of biosensors has resulted in the need to develop new techniques to pattern various sensor elements with high resolution and fast response time. This paper describes the selective deposition of various sensor elements on glass as well as on silicon substrate by combining layer-by-layer electrostatic self-assembly, photolithography and avidin-biotin chemistry. Two methods have been developed for this selective patterning. The first method includes selective UV radiation of a coated photobiotin on glass substrate and covalent bonding of avidin-layered fluorescent nanoparticles to the irradiated areas. The second method involves lithography and self-assembly in a modified lift-off process. In both cases, the epifluorescence microscope images show high spatial resolution of patterned areas and excellent surface coverage. These patterns will be further developed for monitoring cell growth in tissue scaffolds.
Starting Page	1702
Ending Page	1703
File Size	164899
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1106610
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Chemical elements Self-assembly Chemistry Lithography Biosensors Chemical and biological sensors Glass Delay Sensor phenomena and characterization Silicon
Content Type	Text
Resource Type	Article

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