NDLI: In situ synthesis of oligonucleotide arrays by using the molecular stamp method

Content Provider	IEEE Xplore Digital Library
Author	Zuhong Lu Pengfeng Xiao Nongyue He Zhengchun Liu Quanguo He
Copyright Year	2002
Description	Author affiliation: Chien-Shiung Wu Lab., Southeast Univ., Nanjing, China (Zuhong Lu; Pengfeng Xiao; Nongyue He; Zhengchun Liu; Quanguo He)
Abstract	This work developed a technique for in situ synthesis of oligonucleotide arrays on glass surfaces. In this method, based on the standard phosphoramidites chemistry protocol, the coupling was conducted through contacting glass surfaces with a set of molecular stamps on the surfaces of which nucleoside monomer and tetrazole mixed acetonitrile solution were spread. It was shown that 20-mer oligonucleotide on the glass slide was successfully synthesized using the modified polydimethylsiloxane stamps and showed high synthetic efficiency. An effective method has been used to eliminate residual reactive nucleosides on chip with small molecules containing a hydroxyl group. Specific oligonucleotide arrays of four probes including matches and mismatches with the target sequence were fabricated to identify the perfect match and mismatch sequences. Results indicated that the perfect match and mismatch probes hybridized fluorescent signals had clear differences, and may be used to identify and rapidly screen single-nucleotide polymorphisms.
Starting Page	1671
Ending Page	1672
File Size	172080
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1106595
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Glass Probes DNA Sequences Lithography Helium Protocols Laboratories Chemistry Fluorescence
Content Type	Text
Resource Type	Article

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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