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Content Provider | IEEE Xplore Digital Library |
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Author | Cheng-Hsin Chuang Chen-Zhong Li Chen-Che Yeh You-Ming Hsu |
Copyright Year | 2008 |
Description | Author affiliation: Dept. of Mech. Eng., Southern Taiwan Univ., Tainan (Cheng-Hsin Chuang) |
Abstract | Electrorotation has become a very powerful diagnostic technique for measurement of dielectric properties of cells. However, only few papers investigated in the electric-induced rotation of particles in a stationary alternating (AC) electric field instead of a rotating electric field. In this study, a microchip comprised top grounded electrode, flow chamber and bottom chess-type electrode arrays to construct a stationary nonuniform AC electric field for manipulation of cells by dielectrophoretic (DEP) force. We aimed at the discrepancy on electrorotation between metal (Au) and dielectric $(SiO_{2})$ nanoparticles uptaken by the human promyelocytic leukemia cells (HL- 60). As experimental results, both the percentage of cells in rotation and the range of rotational frequency for uptaken-Au-nanoparticles cells were higher and wider than the case of $SiO_{2}$ nanoparticles, respectively. In addition, the cell membrane for a continued rotating cell could be deformed and broken after 30 to 40 minutes. According to the rotational spectra (ROT), the membrane capacitance and membrane conductance of HL-60 cells can be extracted, 9.95 $mF/m^{2}$ and 1460 $S/m^{2},$ respectively. In general, the electrorotation of cells in a stationary AC electric field can be attributed to the highly nonuniform electric field and nonuniform dispersion of nanoparticles within cells; therefore, the electrical properties of uptaken nanoparticles and the aggregation phenomenon have significant influences on resulting electrical torque, the membrane capacitance and membrane conductance of HL-60 cells can be calculated in this method without a rotating electric field excited by poly-phase AC voltages. |
Starting Page | 938 |
Ending Page | 942 |
File Size | 316598 |
Page Count | 5 |
File Format | |
ISBN | 9781424419074 |
DOI | 10.1109/NEMS.2008.4484476 |
Language | English |
Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Publisher Date | 2008-01-06 |
Publisher Place | China |
Access Restriction | Subscribed |
Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Subject Keyword | Nanoparticles Electric fields Biomembranes Nonuniform electric fields Electrodes Cells (biology) Capacitance Dielectric measurements Dielectrophoresis Gold Cell Electrorotation |
Content Type | Text |
Resource Type | Article |
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