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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Alexandrino, J. Sequeira, F. Haddad, C. F. B. Thomé, M. T. C. Lopes, S. |
| Spatial Coverage | Brazil |
| Description | Country affiliation: Brazil Author Affiliation: Thomé MT ( Departamento de Zoologia, Instituto de Biociências, Universidade Estadual Paulista, Rio Claro, SP, Brasil mtcthome@gmail.com.); Alexandrino J ( Departamento de Ciências Biológicas, Universidade Federal de São Paulo, Diadema, SP, Brasil.); Lopes S ( Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal.); Haddad CF ( Departamento de Zoologia, Instituto de Biociências, Universidade Estadual Paulista, Rio Claro, SP, Brasil.); Sequeira F ( Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal.) |
| Abstract | We used pyrosequencing to develop microsatellite markers for the Brazilian four-eyed frog Pleurodema diplolister and tested the microsatellite markers for cross-amplification in its sister Pleurodema alium, which are both endemic species of the Caatinga biome in northeastern Brazil. We used multiplex sets to amplify and genotype 30 individuals of P. diplolister from three different populations and 10 individuals of P. alium from a single population. We successfully amplified 24 loci for P. diplolister, 13 of which we were able to amplify in P. alium. All loci were polymorphic. Significant deviations from the Hardy-Weinberg equilibrium and the presence of null alleles were only consistently detected at one locus (Pleu9). These markers will enable the study of geographic genetic diversity and evolutionary processes in these two Caatinga endemics, and the inclusion of genetic data for conservation planning of the Caatinga biome. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-03-12 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Anura Genetics Genetic Variation Microsatellite Repeats Alleles Animals Genotype Musculoskeletal Abnormalities Osteochondrodysplasias Polymerase Chain Reaction Species Specificity Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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