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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Liu, X. Y. Fu, S. Y. Duan, C. G. Fu, J. J. Yu, H. Q. Gong, S. Mei, Z. Q. Yang, L. Q. |
| Spatial Coverage | China |
| Description | Country affiliation: China Author Affiliation: Mei ZQ ( Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.); Fu SY ( Michael E. DeBakey High School for Health Professions, Houston, TX, USA.); Yu HQ ( Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.); Yang LQ ( Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.); Duan CG ( Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.); Liu XY ( Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.); Gong S ( Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.); Fu JJ ( Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China fujunjiang@hotmail.com.) |
| Abstract | Dimocarpus longan Lour. is an edible and traditional herb in China, commonly referred to as longon. An improved randomly amplified polymorphic DNA (RAPD) protocol was here developed in order to determine the geographical origins of D. longan samples collected from 5 provinces in the southern and southwestern areas of China, including Sichuan, Hainan, Fujian, Guangdong, and Guangxi. Generally, the improved RAPD method generated good fingerprinting of the 5 samples using the selected 17 primers. In particular, primers SBS-A5, SBS-A13, SBS-I9, SBS-I20, SBS-M1, and SBS-Q12 produced distinguishable bands that clearly separated all 5 cultivars, suggesting that there are variations in RAPD genetic sites among the samples. The similarity index ranged from 0.69 to 0.76. The Sichuan and Hainan clades clustered together with a 0.73 similarity index. The Guangxi and Fujian clades clustered together with a 0.76 similarity index, and they formed the sister clade to the Sichuan/Hainan clade with a 0.71 similarity index. The Guangdong clade was in a basal polytomy with a 0.70 similarity index. Based on the abundant DNA polymorphisms, these longan accessions are distinguishable using our improved RAPD technique. Therefore, RAPD analysis is an effective technique in distinguishing the geographical origins of D. longan. Moreover, the improved method could also be employed for a variety of applications including genetic diversity and fingerprinting analyses. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-03-06 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Random Amplified Polymorphic Dna Technique Tracheobionta Classification Genetics Genetic Markers Phylogeny Plants, Medicinal Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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