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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Montoya-Maya, P. H. Macdonald, A. H. H. Schleyer, M. H. |
| Spatial Coverage | Indian Ocean |
| Description | Country affiliation: South Africa Author Affiliation: Montoya-Maya PH ( Oceanographic Research Institute, Durban, South Africa phmontoya@gmail.com.); Macdonald AH ( School of Life Sciences, University of KwaZulu-Natal, Durban, South Africa.); Schleyer MH ( Oceanographic Research Institute, Durban, South Africa.) |
| Abstract | Here, we report the successful cross-species amplification of previously published acroporid microsatellite markers in the coral Acropora austera from the south-western Indian Ocean. This fast-growing species is a major reef-building coral on South African reefs; however, it is the most damaged coral by scuba diving activity, and is known to be very susceptible to coral bleaching. Neither genetic information nor symbiont-free host tissue was available to develop novel microsatellite markers for this species. Cross-species amplification of previously published microsatellite markers was considered as an alternative to overcome these problems. Of the 21 microsatellite markers tested, 6 were reliably amplified, scored, and found to contain polymorphic loci (3-15 alleles). Although microsatellite sequences are believed to be scarce in the Acropora genome because of its small size, the results of this study and previous research indicate that the microsatellite sequences are well conserved across Acropora species. A detailed screening process identified and quantified the sources of error and bias in the application of these markers (e.g., allele scoring error, failure rates, frequency of null alleles), and may be accounted for in the study of the contemporary gene flow of A. austera in the south-western Indian Ocean. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-02-27 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Anthozoa Classification Genetics Microsatellite Repeats Animals Coral Reefs Gene Flow Genetic Loci Indian Ocean Linkage Disequilibrium Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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