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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Reis, J. K. P. Leite, R. C. Camargos, M. F. Stancek, D. Heinemann, M. B. Rajão, D. S. |
| Spatial Coverage | Brazil |
| Description | Country affiliation: Brazil Author Affiliation: Camargos MF ( Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento, Pedro Leopoldo, MG, Brasil.); Rajão DS ( Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.); Leite RC ( Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.); Stancek D ( Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.); Heinemann MB ( Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.); Reis JK ( Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil jenner@ufmg.br.) |
| Abstract | This article reports the selection of bovine leukemia virus (BLV) variants after continuous passage in cell lines or experimental animals. Two wild BLV strains isolated from 2 naturally infected Holstein dairy cows in Brazil (cow codes: 485 and 141) were used for the experimental infection of 1 sheep and FLK cells, and 1 rabbit and CC81 cells. Viral DNA was isolated several months after infection, and env gene nucleotide and amino acid sequences of the 'passaged' variants were compared against the 2 original infecting wild strains. The sequences of the original infecting wild strains were not recovered after their replication in the cell lines or experimental animals. These results indicate that genetic variation occurred after BLV replication in vivo and in vitro, with new variants being selected. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-03-17 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Dna, Viral Genetics Genes, Env Leukemia Virus, Bovine Virus Replication Animals Cell Division Cell Line Pathogenicity Rabbits Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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