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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Xu, G. T. Lv, L. X. Yang, C. Q. Bi, W. R. |
| Description | Country affiliation: China Author Affiliation: Bi WR ( Department of Gastroenterology and Digestive Disease Institute, Tongji Hospital Branch, Tongji University School of Medicine, Shanghai, China.); Xu GT ( Department of Stem Cells Laboratory, Tongji University School of Medicine, Shanghai, China.); Lv LX ( Department of Stem Cells Laboratory, Tongji University School of Medicine, Shanghai, China.); Yang CQ ( Department of Gastroenterology and Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China cqyang@tongji.edu.cn.) |
| Abstract | This study was designed to show whether rat liver epithelial cells could undergo epithelial-mesenchymal transition (EMT), thereby directly contributing to liver fibrosis. The role of the ratio of transforming growth factor-ß1 (TGF-ß1)/bone morphogenetic protein-7 (BMP-7) was evaluated in the progression of EMT or mesenchymal-epithelial transition. Primary rat liver epithelial cells were stimulated with different ratios of TGF-ß1/BMP-7 and examined for evidence of transition to a mesenchymal or epithelial phenotype. Liver sections were labeled to detect antigens associated with liver epithelial cells [E-cadherin (E-cad)], EMT [fibroblast-specific protein-1 (FSP-1), vimentin], myofibroblasts [ -smooth muscle actin ( -SMA)], and intracellular signal-transduction mediated by forming liver fibrosis undergo EMT, resulting in the formation of invasive fibroblasts; this process may be driven or impeded by a response to local TGF-ß1 or BMP-7. BMP-7 downregulated -SMA and phosphorylated Smad2/3. Stimulation of cultured cells with TGF-ß1 induced the expression of pSmad2/3, FSP-1, and -SMA. Stimulation of cultured cells with BMP-7 induced the expression of E-cad. We demonstrated that the cells upregulated E-cad release compared with untreated cells, but TGF-ß1 was different. We found that the equilibrium of the ratio of TGF-ß1/BMP-7 was 1/10. In summary, the mechanism for this process was not determined. Demonstration of the contribution of what the ratio of TGF-ß1/BMP-7 induced to EMT to the chronic liver diseases would provide a new basis for understanding pathogenesis and potential treatment. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-02-20 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Bone Morphogenetic Protein 7 Metabolism Epithelial-mesenchymal Transition Liver Pathology Transforming Growth Factor Beta1 Actins Animals Cadherins Genetics Calcium-binding Proteins Cells, Cultured Disease Models, Animal Epithelial Cells Fibrosis Gene Expression Regulation Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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