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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Haddioui, A. Trifi-Farah, N. Gharbi, M. Zitouna, N. Marghali, S. |
| Description | Author Affiliation: Zitouna N ( Laboratory of Molecular Genetics, Immunology, and Biotechnology.); Marghali S ( Laboratory of Molecular Genetics, Immunology, and Biotechnology.); Gharbi M ( Laboratory of Molecular Genetics, Immunology, and Biotechnology.); Haddioui A ( Laboratory of Plant Genetics and Biotechnology.); Trifi-Farah N ( Laboratory of Molecular Genetics, Immunology, and Biotechnology, neila.trifi@fst.rnu.tn.) |
| Abstract | Six Medicago species were investigated to characterize and valorize plant genetic resources of pastoral interest in Morocco. Samples were obtained from the core collection of the South Australian Research and Development Institute (SARDI). The transferability of single sequence repeat markers of Medicago truncatula was successful with 97.6% efficiency across the five species. A total of 283 alleles and 243 genotypes were generated using seven SSR markers, confirming the high level of polymorphism that is characteristic of the Medicago genus, despite a heterozygosity deficit (HO = 0.378; HE = 0.705). In addition, a high level of gene flow was revealed among the species analyzed with significant intra-specific variation. The unweighted pair group method with arithmetic mean dendrogram generated by the dissimilarity matrix revealed that M. polymorpha and M. orbicularis are closely related, and that M. truncatula is likely the ancestral species. The Pearson correlation index revealed no significant correlations between the geographic distribution of the Moroccan species and genetic similarities, indicating local adaptation of these species to different ecological environments independent of their topographical proximities. The substantial genetic variation observed was likely due to the predominance of selfing species, the relative proximity of prospected sites, human impacts, and the nature of the SARDI core collections, which are selected for their high genetic diversity. The results of this first report on Moroccan Medicago species will be of great interest for establishing strategies aiming at reasonable management and selection programs for local and Mediterranean germplasm in the face of increasing environmental change. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-03-12 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Genetic Variation Medicago Genetics Microsatellite Repeats Phylogeography Alleles Genome, Plant Polymorphism, Genetic Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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