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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zuo, F. Chen, H. Duan, J. |
| Description | Country affiliation: China Author Affiliation: Chen H ( Department of Gynecology and Obstetrics, Zhongnan Hospital of Wuhan University, Wuhan, China hongchendoc@163.com.); Duan J ( Department of Gynecology and Obstetrics, Zhongnan Hospital of Wuhan University, Wuhan, China.); Zuo F ( Department of Gynecology and Obstetrics, Zhongnan Hospital of Wuhan University, Wuhan, China.) |
| Abstract | We examined the ability of mifepristone to reverse the in vitro drug resistance of human cervical cancer cells resistant to mitomycin-C (HeLa/MMC) cells and investigated the mechanism of this effect. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to detect the drug resistance of HeLa/MMC cells and the reversed drug resistance in vitro. Expression levels of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and glucosylceramide synthase (GCS) were measured in HeLa and HeLa/MMC cells. The resistance index of HeLa/MMC cells on MMC was reduced from 5.02 to 1.46 after 10 mg/mL mifepristone exposure. A combination of mifepristone upregulated the Bax/Bcl-2 protein expression ratio and apoptosis in HeLa/MMC cells. GCS expression was significantly higher in HeLa/MMC cells than in HeLa cells (P < 0.01), but distinctly declined in both cell lines after mifepristone application (P < 0.01). Mifepristone reversed the resistance of HeLa/MMC cells to MMC in vitro; the overexpression of the GCS gene and the increased expression of apoptosis-related protein Bcl-2 may play important roles in the formation of multidrug resistance in cervical cancer. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 1 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-02-27 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Drug Resistance, Neoplasm Drug Effects Gene Expression Regulation, Neoplastic Mifepristone Pharmacology Uterine Cervical Neoplasms Pathology Glucosyltransferases Genetics Hela Cells Proto-oncogene Proteins C-bcl-2 Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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