NDLI: Design and Fabrication of In-Vitro Electroporation Chip for Gene Transfection

Content Provider	IEEE Xplore Digital Library
Author	Yen-Liang Yeh Ming-Jyi Jang Cheng-Chi Wang Wen-tai Wei
Copyright Year	2007
Description	Author affiliation: Dept. of Autom. & Control Eng., Far East Univ. (Yen-Liang Yeh; Ming-Jyi Jang; Cheng-Chi Wang; Wen-tai Wei)
Abstract	This paper presents the design and fabrication of an array-type bio-chip with a novel 3D electrode structure for gene delivery. The bio-chip enables the processes of gene delivery and cell culturing to be performed in a single device. The genes are introduced into the host cell via a process of electroporation induced by the application of a transient electric field to the electrodes of the bio-chip. The 3D electrode structure for gene delivery can cause the position of the positive and negative electrode is different. When the position of the positive and negative electrode is different, the electroporation and electrophoretic forces are applied at the same time. The bio-chip comprises three major components, namely a reaction chamber, a cell accommodating structure, and a 3D interdigitated electrode arrangement. The chip is fabricated using conventional micro-electro mechanical systems (MEMS) techniques. The study establishes two different types of foundation plates the array type gene delivery to breed the chip, and use electricity pulse electric field test, may effectively improve strengthens the electric field power, enhances the chip transformation to breed the function. This result indicates that the efficiency of 3D structure bio-chip is high than the plane structure by using water testing.
Starting Page	294
Ending Page	298
File Size	5364566
Page Count	5
File Format	PDF
ISBN	1424406102
DOI	10.1109/NEMS.2007.352030
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-01-16
Publisher Place	Thailand
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fabrication In vitro Electrodes DNA Proteins Mechanical systems Micromechanical devices Cells (biology) Design engineering Testing Electrophoresis Gene delivery Micro-Electro Mechanical System (MEMS) Electroporation
Content Type	Text
Resource Type	Article

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