NDLI: A Single PDMS Based Chip for E. Coli Bacteria Lysis, DNA Extraction and Amplification

Content Provider	IEEE Xplore Digital Library
Author	Yuji Wang Qinghui Jin Jianlong Zhao
Copyright Year	2007
Description	Author affiliation: Shanghai Inst. of Microsyst. & Inf. Technol., Chinese Acad. of Sci., Beijing (Yuji Wang; Qinghui Jin; Jianlong Zhao)
Abstract	An integrated microfluidic device with on-chip performance of cell lysis, DNA extraction and PCR amplification is achieved to realize the total analysis from cell lysis to DNA amplification on a single polydimethylsiloxane (PDMS) chip. Using multilayered PDMS patterning and solid phase extraction (SPE) techniques, DNA is extracted by magnetic beads (MBs) from the cell lysate prepared by a two-step thermal regime. After DNA extraction, instead of traditional DNA amplification requiring washing MBs out, a non-elution on-chip PCR is performed with DNA captured by MBs serving directly as the template. The procedure from DNA extraction to amplification is simplified and can be realized on one chip without washing MBs out. The feasibility of realizing whole process on a single chip is verified by the gel electrophoresis result. This study shows an approach towards the integrated microsystem which is capable of comprehensive genetic analysis.
Starting Page	458
Ending Page	461
File Size	3943926
Page Count	4
File Format	PDF
ISBN	1424406102
DOI	10.1109/NEMS.2007.352058
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-01-16
Publisher Place	Thailand
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microorganisms DNA Microfluidics Magnetic analysis Solids Biological materials Costs Silicon Glass Polymers PDMS single chip magnetic beads uTAS
Content Type	Text
Resource Type	Article

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