NDLI: Factors Influencing the Properties of Rifampicin Liposome and Applications for Dry Powder Inhaler

Content Provider	IEEE Xplore Digital Library
Author	Changsan, N. Srichana, T.
Copyright Year	2007
Description	Author affiliation: Dept. of Pharm. Technol., Prince of Songkla Univ. (Changsan, N.; Srichana, T.)
Abstract	RIF encapsulated liposome vesicles were prepared by chloroform film method followed by freeze drying technique to obtain a dry powder for aerosol delivery. The freeze drying conditions were designed according to the DSC results of the liposome suspension. Three sugars (mannitol, lactose and trehalose) were used as a cryoprotectant of liposome dry powder. NR 8383 cell line was used to determine immunological activation and toxicity of liposome products when LPS from E.coli was used as a positive control. High cholesterol content in the formulation created higher rigid bilayer membrane of liposome vesicle than the lower cholesterol content formulation thus provided a better physical stability. The lipid content had influenced on degree of encapsulation, higher lipid content in formulation produced higher % encapsulation. Mannitol was a suitable sugar for this dry powder aerosol when it provided a free flowing powder with an MMAD less than 5 mum (3.35 mum). In addition, RIF in liposome dry powder form showed better chemical stability than in suspension form after they were kept for 6 weeks both at 4degC and room temperature. The reconstituted liposome powder in PBS pH 7.4 gave the encapsulation about 22%. The products did not cause toxicity to the cell line and did not activate immune responses since the cell produced very low level of toxic cytokines (IL-lbeta and TNF-alpha) when compared to LPS. This indicates that the particles are able to reach alveoli without stimulation of immunological response and safe to alveolar macrophage.
Starting Page	1104
Ending Page	1109
File Size	7591717
Page Count	6
File Format	PDF
ISBN	1424406102
DOI	10.1109/NEMS.2007.352211
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-01-16
Publisher Place	Thailand
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Powders Drugs Immune system Lungs Aerosols Encapsulation Antibiotics Stability Lipidomics Human immunodeficiency virus Liposome Rifampicin Dry powder inhaler
Content Type	Text
Resource Type	Article

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