NDLI: Cell array fluidic channel integrated with electrodes for cell-based multiple chemical sensing

Content Provider	IEEE Xplore Digital Library
Author	Misawa, N. Lee, H.J. Mitsuno, H. Kanzaki, R. Sawada, K.
Copyright Year	2013
Description	Author affiliation: EIIRIS, Toyohashi Univ. of Technol., Toyohashi, Japan (Misawa, N.) \|\| RCAST, Univ. of Tokyo, Tokyo, Japan (Mitsuno, H.; Kanzaki, R.) \|\| ICG, Toyohashi Univ. of Technol., Toyohashi, Japan (Lee, H.J.; Sawada, K.)
Abstract	This paper describes a hybrid device of a fluidic channel and electrodes for detection of membrane potential changes of Xenopus laevis oocytes (Xenopus oocytes). The fluidic device is composed poly(methyl methacrylate) and a silicon substrate that has needle-shaped structures for electrodes of two-electrode voltage clamping method that can measure ion flows across plasma membrane using a feedback amplifier and two electrodes inserted into a cell. To construct the fluidic device integrated with electrodes, we simulated and calculated the fluid behavior in the fluidic device for Xenopus oocytes array and investigated the flow rate for electrodes insertion into Xenopus oocytes. Using the device, we successfully demonstrated Xenopus oocytes array and detected the oocyte responses to $K^{+}$ stimulus as a model of chemical sensing.
Starting Page	2451
Ending Page	2454
File Size	4483890
Page Count	4
File Format	PDF
ISBN	9781467359832
ISSN	21641641
DOI	10.1109/Transducers.2013.6627302
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-06-16
Publisher Place	Spain
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Arrays Chemicals Charge carrier processes Biomembranes Substrates Proteins Two-electrode voltage clamping method Chemical sensor Chemical receptor Xenopus laevis oocyte Cell-array Fluidic device
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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