NDLI: Microfluidic solutions for miniaturization, integration, automation and parallelization of tests on commercially available instruments

Content Provider	IEEE Xplore Digital Library
Author	Zengerle, R. Mark, D. Kosse, D. Roth, G. von Stetten, F.
Copyright Year	2011
Description	Author affiliation: HSG-IMIT, Wilhelm-Schickard-Straße 10, D-78052 Villingen-Schwenningen, Germany (Zengerle, R.; Mark, D.; Kosse, D.; Roth, G.; von Stetten, F.)
Abstract	We demonstrate that Lab-on-a-Chip implementations can be designed for operation in common commercialized instruments like laboratory centrifuges, DVD drives, or real-time PCR cyclers. On the one hand this approach could render the expensive development of tailor-made instruments superfluous and could significantly reduce market entry barriers for developing Lab-on-a-Chip solutions. On the other hand this provides instrument companies with the opportunity to upgrade their devices e.g. by integrating complex sample preparation protocols prior to the detection for which the instruments are designed. We demonstrate this approach for the following applications: • automated DNA-purification operated on a standard laboratory centrifuges with 50% yield compared to gold standards, • DNA pre-amplification, aliquoting, and real-time PCR operated on a slightly modified real-time thermocycler (Rotor-Gene) with < 10 copy sensitivity, • isothermal DNA amplification by recombinase polymerase amplification with < 20 copy sensitivity and a time-to-result of less than 15 minutes, also operated on the Rotor-Gene platform, • hematocrit measurement of less than 10 µL of whole blood in a DVD drive.
Starting Page	12
Ending Page	15
File Size	962556
Page Count	4
File Format	PDF
ISBN	9781457701573
e-ISBN	9781457701566
DOI	10.1109/TRANSDUCERS.2011.5969897
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-06-05
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microfluidics DNA Instruments Laboratories DVD Real time systems Purification hematocrit Lab-on-a-chip standard instruments microfluidics microfluidic platforms PCR RPA DNA purification
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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