NDLI: Isothermal Recombinase Polymerase Amplification (RPA) of E. coli gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms

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Isothermal Recombinase Polymerase Amplification (RPA) of E. coli gDNA in Commercially Fabricated PCB-Based Microfluidic Platforms

Content Provider	MDPI
Author	Georgoutsou-Spyridonos, Maria Filippidou, Myrto Kaprou, Georgia D. Mastellos, Dimitrios C. Chatzandroulis, Stavros Tserepi, Angeliki
Copyright Year	2021
Description	Printed circuit board (PCB) technology has been recently proposed as a convenient platform for seamlessly integrating electronics and microfluidics in the same substrate, thus facilitating the introduction of integrated and low-cost microfluidic devices to the market, thanks to the inherent upscaling potential of the PCB industry. Herein, a microfluidic chip, encompassing on PCB both a meandering microchannel and microheaters to accommodate recombinase polymerase amplification (RPA), is designed and commercially fabricated for the first time on PCB. The developed microchip is validated for RPA-based amplification of two E. coli target genes compared to a conventional thermocycler. The RPA performance of the PCB microchip was found to be well-comparable to that of a thermocycler yet with a remarkably lower power consumption (0.6 W). This microchip is intended for seamless integration with biosensors in the same PCB substrate for the development of a point-of-care (POC) molecular diagnostics platform.
Starting Page	1387
e-ISSN	2072666X
DOI	10.3390/mi12111387
Journal	Micromachines
Issue Number	11
Volume Number	12
Language	English
Publisher	MDPI
Publisher Date	2021-11-12
Access Restriction	Open
Subject Keyword	Micromachines Microbiology Pcb Technology Dna Amplification Rpa Microfluidics Microheaters E. Coli Molecular Diagnostics
Content Type	Text
Resource Type	Article

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