NDLI: Si nano-pillars for measuring traction force exerted by filopodia

Content Provider	IEEE Xplore Digital Library
Author	Uijin Jung Kan, T. Kuwana, K. Matsumoto, K. Shimoyama, I.
Copyright Year	2011
Description	Author affiliation: The University of Tokyo, JAPAN (Uijin Jung; Kan, T.; Kuwana, K.; Matsumoto, K.; Shimoyama, I.)
Abstract	This paper describes a Si nano-pillars array for measuring traction forces of one singular filopodium (100–300 nm in diameter). We fabricated the Si nano-pillar which was 110 nm in diameter and 1.5 µm in length. Fibroblasts were cultured on the Si nano-pillar array. The filopodium of fibroblast adhered to pillar and dragged it. We calculated the traction force from the deflection of the pillar. When spring constants of Si nano-pillars were 1.7 N/m, 4.1 N/m and 6.6 N/m, averaged traction forces were 580 nN, 1 µN and 2.3 µN, respectively. The cells exerted the large forces with an increase in the stiffness of the pillar. We confirmed that the Si nano-pillar array was able to be used for measuring the traction forces of filopodium.
Starting Page	2754
Ending Page	2757
File Size	3210590
Page Count	4
File Format	PDF
ISBN	9781457701573
e-ISBN	9781457701566
DOI	10.1109/TRANSDUCERS.2011.5969359
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-06-05
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Silicon Force Arrays Substrates Nanobioscience Force measurement Fibroblasts MEMS Filopodia Traction force Si nano-pillar Cell migration
Content Type	Text
Resource Type	Article

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