NDLI: Analysis of the contraction of fibroblast-collagen gels and the traction force of individual cells by a novel elementary structural model

Content Provider	IEEE Xplore Digital Library
Author	Feng, Z. Wagatsuma, Y. Kobayashi, S. Kosawada, T. Sato, D. Nakamura, T. Kitajima, T. Umezu, M.
Copyright Year	2013
Description	Author affiliation: Integrative Biosci. & Biomed. Eng., Waseda Univ., Tokyo, Japan (Umezu, M.) \|\| Yamagata Univ., Yamagata, Japan (Feng, Z.; Wagatsuma, Y.; Kobayashi, S.; Kosawada, T.; Sato, D.; Nakamura, T.) \|\| Dept. of Electron. Syst. Eng., Malaysia-Japan Int. Inst. of Technol., Kuala Lumpur, Malaysia (Kitajima, T.)
Abstract	Based on the experimental data of the contraction ratio of fibroblast-collagen gels with different initial collagen concentrations and cell numbers, we analyzed the traction force exerted by individual cells through a novel elementary structural model. We postulate that the mechanical mechanism of the gel contraction is mainly because that populated cells apply traction force to some of the surrounding collagen fibrils with such proper length potential to be pulled straight so as to be able to sustain the traction force; this traction induce the cells moving closely to each other and consequently compact the fibrillar network; the bending force of the fibrils in turn resists the movement. By employing fiber packing theory for random fibrillar networks and network alteration theory, the bending force of collagen fibrils was deduced. The traction force exerted by individual fibroblasts in the gels was balanced by the bending force and the resistance from interstitial fluid since inertial force can be neglected. The maximum traction force per cell under free floating condition is in the range of 0.27-9.02 nN depending on the initial collagen concentration and populated cell number. The most important outcome of this study is that the traction force of individual cells dynamically varies under different gel conditions, whereas the adhesion force between cell and individual fibrils is relatively converging and stable.
Starting Page	6232
Ending Page	6235
File Size	785749
Page Count	4
File Format	PDF
ISBN	9781457702167
ISSN	1557170X
DOI	10.1109/EMBC.2013.6610977
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-07-03
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Force Adhesives Fibroblasts Optical fiber networks Optical fiber theory Fluids Elasticity
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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