NDLI: Effect of low-intensity pulsed ultrasound on the nano-mechanical properties of cholesterol-manipulated cells

Content Provider	IEEE Xplore Digital Library
Author	Wen-Hsuan Lai Jiunn-Der Liao Shyh-Hau Wang
Copyright Year	2012
Description	Author affiliation: Dept. of Comput. Sci. & Inf. Eng. & Inst. of Med. Inf., Nat. Cheng Kung Univ., Tainan, Taiwan (Wen-Hsuan Lai; Shyh-Hau Wang) \|\| Dept. of Mater. Sci. & Eng., Nat. Cheng Kung Univ., Tainan, Taiwan (Jiunn-Der Liao)
Abstract	The mechanical properties of cells have been verified to correlate to human diseases and aging as well as to various cellular physiological processes, including proliferation, migration, and differentiation. It thus is essential to investigate responses of cells corresponding to chemical and physical stimulations for cellular mechanics studies and other applications. Moreover, measurement of the stiffness of an interested cell is of great importance to comprehend the cellular response with respect to a specific biophysical or biochemical alteration. Hence, current study aims to investigate the effect of low-intensity pulsed ultrasound (LIPUS) on nano-mechanical properties of cholesterol-manipulated cells. The NIH-3T3 fibroblasts were firstly incubated in cholesterol depletion solution. The cells were then insonified by a 1 MHz LIPUS for three minutes during the process of cholesterol restoration. In situ observation on the variations of living cells adhered on the glass substrate was monitored continuously. The cellular morphological changes were recorded for 8 hours. The nanomechanical properties of cells were detected with nanoindentation by measuring the cellular membrane of a single cell. The cell properties seemed to be unchanged when adding with serum. On the other hand, the cholesterol depletion tended to lead a majority of cells to retract and become rounding corresponding to the increase of incubation time. The complications may be alleviated with the insonification of LIPUS. The elastic properties of those cholesterol-manipulated cells following LIPUS insonification tended to be improved. This study demonstrates that the variations of nano-mechanical property of cell membrane during cholesterol restoration and LIPUS insonification may be sensitively detected using the depth-sensing nano-indentation technique. The LIPUS insonification may be beneficial to the restoration of elastic properties of cholesterol manipulated cells.
Starting Page	600
Ending Page	603
File Size	430368
Page Count	4
File Format	PDF
ISBN	9781467345613
ISSN	19485719
e-ISBN	9781467345620
DOI	10.1109/ULTSYM.2012.0149
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-10-07
Publisher Place	Germany
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Biomembranes Cells (biology) Nanobioscience Mechanical factors Image restoration Ultrasonic imaging Lipidomics cholesterol-manipulated low-intensity pulsed ultrasound nano-indentation
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in