NDLI: Surface contact of bound targeted microbubbles

Content Provider	IEEE Xplore Digital Library
Author	Kooiman, K. Kokhuis, T.J.A. Skachkov, I. Bosch, J.G. van der Steen, A.F.W. van Cappellen, W.A. de Jong, N.
Copyright Year	2012
Description	Author affiliation: Dept. of Biomed. Eng., Erasmus MC, Rotterdam, Netherlands (Kooiman, K.; Kokhuis, T.J.A.; Skachkov, I.; Bosch, J.G.; van der Steen, A.F.W.; de Jong, N.) \|\| Dept. of Reproduction & Dev., Erasmus MC, Rotterdam, Netherlands (van Cappellen, W.A.)
Abstract	For molecular imaging using ultrasound contrast agents, targeted microbubbles are designed with specific ligands linked to the coated shell. Research is ongoing to determine the binding force of targeted microbubbles and to distinguish bound from unbound targeted microbubbles using ultrasound. For this, the actual surface of the targeted microbubbles that binds to a pathology is important. This study focuses on determining the surface contact of bound targeted microbubbles by fluorescence microscopy. Biotinylated lipid-coated microbubbles (3-7 μm in diameter) with either DSPC or DPPC as the main lipid were targeted to a streptavidin-coated surface. The binding area of targeted microbubbles was found to be 6 ± 4% of the total microbubble surface for microbubbles with DSPC as the main lipid (n=22) and 11 ± 4% for microbubbles with DPPC as the main lipid (n=24). The difference can be explained by the heterogeneous distribution of the ligand for DSPC microbubbles whereas the ligand is homogeneously distributed for DPPC microbubbles. These findings can be used to improve the binding of targeted microbubbles and for the ongoing research to distinguish bound from unbound microbubbles.
Starting Page	2161
Ending Page	2163
File Size	956627
Page Count	3
File Format	PDF
ISBN	9781467345613
ISSN	19485719
e-ISBN	9781467345620
DOI	10.1109/ULTSYM.2012.0539
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-10-07
Publisher Place	Germany
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Lipidomics Surface treatment Ultrasonic imaging Molecular imaging Fluorescence Coatings molecular imaging ultrasound contrast agent ligand distribution targeted microbubbles surface contact
Content Type	Text
Resource Type	Article

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