NDLI: Development of a specific tracer for metabolic imaging of alveolar echinococcosis: A preclinical study

Content Provider	IEEE Xplore Digital Library
Author	Porot, C. Knapp, J. Junhua Wang Germain, S. Camporese, D. Seimbille, Y. Boulahdour, H. Vuitton, D.A. Gottstein, B. Blagosklonov, O.
Copyright Year	2014
Description	Author affiliation: WHO Collaborating Centre for Prevention & Treatment of Human Echinococcosis, Univ. of Franche-Comte, Besancon, France (Porot, C.; Knapp, J.; Vuitton, D.A.; Blagosklonov, O.) \|\| Inst. of Parasitology, Univ. of Franche-Comte, Bern, Switzerland (Junhua Wang) \|\| Adv. Accel. Applic. SA, St. Genis Pouilly, France (Camporese, D.) \|\| Inst. of Parasitology, Univ. of Bern, Bern, Switzerland (Gottstein, B.) \|\| Univ. Hosp., Univ. of Geneva, Geneva, Switzerland (Germain, S.; Seimbille, Y.) \|\| Univ. Hosp., Univ. of Franche-Comte, Besancon, France (Boulahdour, H.)
Abstract	Positron emission tomography (PET)-computed tomography (CT) using [18F]-fluorodeoxyglucose (FDG) (FDG-PET/CT) is a valuable method for initial staging and follow up of patients with alveolar echinococcosis (AE). However, the cells responsible for FDG uptake have not been clearly identified. The main goal of our study was to evaluate the uptake of PET tracers by the cells involved in the host-parasite reaction around AE lesions as the first step to develop a specific PET tracer that would allow direct assessment of parasite viability in AE. Candidate molecules ([18F]-fluorotyrosine (FET), [18F]-fluorothymidine (FLT), and [18F]-fluorometylcholine (FMC), were compared to FDG by in vitro studies on human leukocytes and parasite vesicles. Our results confirmed that FDG was mainly consumed by immune cells and showed that FLT was the best candidate tracer for parasite metabolism. Indeed, parasite cells exhibited high uptake of FLT. We also performed PET/CT scans in mice infected intraperitoneally with E. multilocularis metacestodes. PET images showed no FDG or FLT uptake in parasitic lesions. This preliminary study assessed the metabolic activity of human leukocytes and AE cells using radiolabeling. Future studies could develop a specific PET tracer for AE lesions to improve lesion detection and echinococcosis treatment in patients. Our results demonstrated that a new animal model is needed for preclinical PET imaging to better mimic human hepatic and/or periparasitic metabolism.
Sponsorship	IEEE Eng. Med. Biol. Soc.
Starting Page	5587
Ending Page	5590
File Size	799409
Page Count	4
File Format	PDF
ISBN	9781424479290
ISSN	1557170X
DOI	10.1109/EMBC.2014.6944893
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-08-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Positron emission tomography Lesions Mice In vitro Computed tomography
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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